Alkaloids in plants and root cultures of Atropa belladonna overexpressing putrescine N‐methyltransferase
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چکیده
منابع مشابه
Alkaloids in plants and root cultures of Atropa belladonna overexpressing putrescine N-methyltransferase.
Putrescine N-methyltransferase (PMT) is the first alkaloid-specific enzyme for nicotine and tropane alkaloid formation. The pmt gene from Nicotiana tabacum was fused to the CaMV 35S promoter and integrated into the Atropa belladonna genome. Transgenic plants and derived root cultures were analysed for gene expression and for levels of alkaloids and their precursors. Scopolamine, hyoscyamine, tr...
متن کاملImprovement of tropane alkaloids production in hairy root cultures of Atropa belladonna by overexpressing pmt and h6h genes
Atropa belladonna L. is the commercial plant to produce tropane alkaloids including hyoscyamine and scopolamine, which are widely used as anticholinergic agents. In the present study, the genes encoding the key enzymes including putrescine N-methyltransferase (PMT) and hyoscyamine 6β-hydroxylase (H6H) were simultaneously overexpressed in transgenic hairy root cultures of A. belladonna. All of t...
متن کاملOverexpression of tropinone reductases alters alkaloid composition in Atropa belladonna root cultures.
The medicinally applied tropane alkaloids hyoscyamine and scopolamine are produced in Atropa belladonna L. and in a small number of other Solanaceae. Calystegines are nortropane alkaloids that derive from a branching point in the tropane alkaloid biosynthetic pathway. In A. belladonna root cultures, calystegine molar concentration is 2-fold higher than that of hyoscyamine and scopolamine. In th...
متن کاملData for characterizing genetically transformed cultures of Atropa belladonna L
in journal: 1. Bálványos, I., László, I., Vida, K., Hank, H., Kursinszki, L., Tóth, E., Sz ke, É.(2001): Effect of magnesium on the genetically modified Atropa belladonna cultures. Magnesium Research 14, 309-310. IF: 0,689 2. Hank H., Bálványos I., Kursinszki L., Tóth E., Sz ke É. (2003): A táptalaj összetételének hatása transzgénikus Atropa belladonna kultúrák alkaloid produkciójára. Gyógyszer...
متن کاملIdentification and determination of different alkaloids from Atropa belladonna L. by Gas chromatography method
Background & Aim: A. belladonna (family: Solanaceae) is one of important pharmaceutical plants which contain tropane alkaloids. Tropane alkaloids are distinct group of secondary metabolites of the Solanaceae family. The most important alkaloids of A. belladonna are atropine and hyoscine that are used extensively because of their medicinal properties. There...
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ژورنال
عنوان ژورنال: Journal of Experimental Botany
سال: 2003
ISSN: 1460-2431,0022-0957
DOI: 10.1093/jxb/erg227